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KMID : 0191120150300030233
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Journal of Korean Medical Science
2015 Volume.30 No. 3 p.233 ~ p.239
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Eupatilin Ameliorates Collagen Induced Arthritis
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Kim Ju-Ryun
Kim Young-Kyun
Yi Hyo-Ju
Jung Hye-Rin
Yeri Alice Rim
Park Na-Rae
Jung Seung-Min
Park Sung-Hwan
Ju Ji-Hyeon
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Abstract
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Eupatilin is the main active component of DA-9601, an extract from Artemisia. Recently, eupatilin was reported to have anti-inflammatory properties. We investigated the anti-arthritic effect of eupatilin in a murine arthritis model and human rheumatoid synoviocytes. DA-9601 was injected into collagen-induced arthritis (CIA) mice. Arthritis score was regularly evaluated. Mouse monocytes were differentiated into osteoclasts when eupatilin was added simultaneously. Osteoclasts were stained with tartrate-resistant acid phosphatase and then manually counted. Rheumatoid synoviocytes were stimulated with TNF-α and then treated with eupatilin, and the levels of IL-6 and IL-1β mRNA expression in synoviocytes were measured by RT-PCR. Intraperitoneal injection of DA-9601 reduced arthritis scores in CIA mice. TNF-α treatment of synoviocytes increased the expression of IL-6 and IL-1β mRNAs, which was inhibited by eupatilin. Eupatilin decreased the number of osteoclasts in a concentration dependent manner. These findings, showing that eupatilin and DA-9601 inhibited the expression of inflammatory cytokines and the differentiation of osteoclasts, suggest that eupatilin and DA-9601 is a candidate anti-inflammatory agent.
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KEYWORD
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Eupatilin, Arthritis, Experomental, Arthritis, Rheumatoid, DA-9601
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